Megjelenés éve: Kivonat: In rodents, moderate caloric restriction CR without malnutrition exerts significant cerebrovascular protective effects, improving cortical microvascular density and endothelium-dependent vasodilation, but the underlying cellular mechanisms remain elusive.
We found an age-related increase in cellular and mitochondrial oxidative stress, which is prevented by CR. Expression of miR was up-regulated in aged Pro anti aging and overexpression of miR significantly decreased expression of Nrf2 in cells derived from both young animals and aged CR rats.
We found that CR prevents age-related impairment of angiogenic processes, including cell proliferation, adhesion to collagen and formation of capillary-like structures and inhibits apoptosis in CMVECs.
CR also exerts significant anti-inflammatory effects, preventing age-related increases in the transcriptional activity of NF-kappaB and age-associated pro-inflammatory shift in the endothelial secretome. Characterization of CR-induced changes in miRNA expression suggests that they likely affect several critical functions in endothelial cell homeostasis.
Collectively, we find that CR confers persisting anti-oxidative, pro-angiogenic and anti-inflammatory cellular effects preserving a youthful phenotype in rat cerebromicrovascular endothelial cells, suggesting that through these effects CR may improve pro anti aging function and prevent vascular cognitive impairment.